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[db-038] 3B41 : ラット胎仔下垂体前葉の細胞増殖: BrdUとLHbの二重免疫染色による検討



##Begin
#Z 3B41
#A ラット胎仔下垂体前葉の細胞増殖: BrdUとLHbの二重免疫染色による検討
#B Proliferation of pituitary LH cells through fetal and neonatal period
#E1 谷口/豊
#F1 たにぐち/ゆたか
#G1 Taniguchi/Yutaka
#J1 金沢医科大学解剖学第二講座
#K1 かなざわいかだいがくかいぼうがくだいにこうざ
#L1 2nd Department of Anatomy, Kanazawa Medical University
#E2 木南/利栄子
#F2 こみなみ/りえこ
#G2 Kominami/Rieko
#J2 金沢医科大学解剖学第二講座
#K2 かなざわいかだいがくかいぼうがくだいにこうざ
#L2 2nd Department of Anatomy, Kanazawa Medical University
#E3 安高/悟
#F3 やすたか/さとる
#G3 Yasutaka/Satoru
#J3 金沢医科大学解剖学第二講座
#K3 かなざわいかだいがくかいぼうがくだいにこうざ
#L3 2nd Department of Anatomy, Kanazawa Medical University
#E4 瓦井/康之
#F4 かわらい/やすゆき
#G4 Kawarai/Yasuyuki
#J4 金沢医科大学解剖学第二講座
#K4 かなざわいかだいがくかいぼうがくだいにこうざ
#L4 2nd Department of Anatomy, Kanazawa Medical University
#D
#M ラット,下垂体,LH(黄体化ホルモン), 免疫組織化学,発生,増殖
#N rat, pituitary, LH, immunocytochemistry, ontogenesis, proliferation
#T Materials & Methods: Rat fetuses (17.5-21.5 days of gestation) and
 neonates (3, 7, 14 and 28 days) were used. Bromodeoxyuridine (BrdU,
100mg/KgBW) was i.p. injected to the mother rat or neonates 2 hours
prior
to sacrifice and the pituitaries were fixed with Bouin's fluid.
Paraffin sections were consecutively immuno-stained with anti-BrdU and
anti-ovine LHb and
the number of positive cells were counted.
Results: The cells labeled with BrdU were around 600-800/mm2
through the fetal period and 3 days after birth and then gradually
decreased to 146/mm2 at 28 days. The cells labeled with anti-LHb were
first
detected at 18.5 days and remained below 100/mm2 throughout the fetal
period but they rapidly increased after birth to reach 800/mm2 at 28
days.
The cells double-labeled with both anti-sera were detected at 3-14 days
after birth. But they comprised only 3% or less of total gonadotrophs
so it
seemed that the majority of go-nadotrophs were derived from
differentiation of some kind of stem cells with little contribution by
mitoses of existing
gonadotrophs.
#Last_modified 97.03.25-12:23
#Return_path yuh-tani@kanazawa-med.ac.jp
#Sequence_number   38
##End

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