[db-027] 1A09 : 細胞骨格によるexocytosis-endocytosis couplingの調節機構

• To: db-abst@sapmed.ac.jp
• Subject: [db-027] 1A09 : 細胞骨格によるexocytosis-endocytosis couplingの調節機構
• From: segawa@kitasato-u.ac.jp
• Date: Fri, 21 Mar 1997 13:37:23 +0900 (JST)

##Begin
#Z 1A09
#A 細胞骨格によるexocytosis-endocytosis couplingの調節機構
#B Regulation of exocytosis-endocytosis coupling by cytoskeleton
#E1 瀬川/彰久
#F1 せがわ/あきひさ
#G1 Segawa/Akihisa
#J1 北里大学医学部解剖
#K1 きたさとだいがくいがくぶかいぼう
#L1 Department of Anatmoy, School of Medicine, Kitasato University
#E2 山科/正平
#F2 やましな/しょうへい
#G2 Yamashina/Shohei
#J2 北里大学医学部解剖
#K2 きたさとだいがくいがくぶかいぼう
#L2 Department of Anatmoy, School of Medicine, Kitasato University
#D Regulation of exocytosis-endocytosis coupling by cytoskeleton. Akihisa Segawa & Shohei Yamashina (Dept Anat, Sch Med, Kitasato Univ, Sagamihara)
 Roles of microfilaments in the regulation of post-fusion process of exocytosis were studied in parotid and submand-ibular acinar cells of rats and humans. b-stimulation with isoproterenol evoked the appearance of exocytotic profiles, which under the confocal microscope were of granule-size disappearing within a few minutes. On/around the fused membranes, F-actin fluorescence was detected and coated pits/vesicles were abundant. Muscarinic agonist carbachol also induced exocytosis, but it exhibited neither F-actin fluorescence nor coated pits/vesicles around the fused membranes, which were far beyond the granule-size remained on cell surface for longer than 30 min. Treatment of isoproterenol-stimulated cells further with cytochalasin D or carbachol resulted in the disruptive changes of F-actin and coated pits/vesicles, and the exocytotic profiles greatly enlarged. Post-fusion process of salivary exocytosis is thus considered to be regulated by microfilaments.
(Collaboration with Prof A Riva, Cagliari University, Italy)
#M 唾液腺、開口分泌、細胞骨格
#N salivary gland, exocytosis, cytoskeleton
#Last_modified 97.03.21-13:37
#Return_path segawa@kitasato-u.ac.jp
#Sequence_number   27
##End

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