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[db-072] : ラット胃底腺ペプシンノーゲン産生細胞の個体発生

##Begin
#1E13
#A ラット胃底腺ペプシンノーゲン産生細胞の個体発生
#B Ontogeny of pepsinogen 1 producing cells in rat fundic gland
#E1 戈/応濱
#F1 げ/いんびん
#G1 Ge/Yingbin
#J1 鹿児島大学医学部第二解剖
#K1 かごしまだいがくいがくぶだいにかいぼう
#L1 Department of Anatomy, Facolty of Medicine, Kagoshima university
#E2 大森/淳
#F2 おおもり/じゅん
#G2 Ohmori/Jun
#J2 鹿児島大学医学部第二解剖
#K2 かごしまだいがくいがくぶだいにかいぼう
#L2 Department of Anatomy, Facolty of Medicine, Kagoshima university
#E3 津山/新一郎
#F3 つやま/しんいちろう
#G3 Tsuyama/Shinichiro
#J3 鹿児島大学医学部第二解剖
#K3 かごしまだいがくいがくぶだいにかいぼう
#L3 Department of Anatomy, Facolty of Medicine, Kagoshima university
#E4 楊/冬華
#F4 やん/どんか
#G4 Yang/Donghua
#J4 鹿児島大学医学部第二解剖
#K4 かごしまだいがくいがくぶだいにかいぼう
#L4 Department of Anatomy, Facolty of Medicine, Kagoshima university
#E5 村田/長芳
#F5 むらた/ふさよし
#G5 Murata/Fusayoshi
#J5 鹿児島大学医学部第二解剖
#K5 かごしまだいがくいがくぶだいにかいぼう
#L5 Department of Anatomy, Facolty of Medicine, Kagoshima university
#M ペプシンノーゲン、胃底腺、個体発生、ラット、免疫組織化学、ハイブリダイ
ゼション
#N pepsinogen, fundic gland, ontogeny, rat immunohistochemstry,
 in situ hybridization
#T
 Ontogeny of pepsinogen 1 producing cells in rat fundic gland
Y-B Ge, J. Ohmori, S. Tsuyama, D-H Yang and F. Murata (Department of
Anatomy, Faculty of Medicine, Kagoshima University, Kagoshima 890, Japan)
The ontogeny of pepsinogen-1 producing cells in rat fundic gland has
been studied by means of light and electron microscopic
immunohistochemistry and in situ hybridization method. Wistar rat fetuses
(at 17.5, 18.5, 19.5, 20.5 and 21.5 days of gestation), suckings
(postnatal days 0.5, 3.5, 7 and 14), weanlings (postnatal days 21 and 28)
and adults (postnatal day 56) were used in this experiment.
For light microscopic immunohistochemistry, rat fundic glands were
fixed in Bouin's solution and embedded in paraffin. A biotin conjugated
goat anti-rabbit IgG and HRP conjugated streptavidin were used to detect
the rat pepsinogen 1 localization. For electron microscopic
immunocytochemistry, rat fundic glands were fixed in 1/2 Karnovsky 's
solution and embedded in Lowicryl K4M resin. Colloidal gold conjugated
protein A was used to detect rat pepsinogen 1 localization. For in situ
hybridization, paraformaldehyde fixed and paraffin embedded tissues were
used. Tissues were hybridized at 50 ℃overnight. An alkaline phosphatase con
jugated goat anti-digoxigenin antibody was used to detect pepsinogen 1
mRNA.
In adult rats, pepsinogen is produced by chief cells, intermediate
mucous neck/chief cells and mucous neck cells. Pepsinogen 1 producing cells
appeared as early as embryo did at 18.5 days. mRNA of pepsinogen was
discernible in above mentioned three cell types. Our immunohistochemical
and in situ hybridization results strongly indicate that a mucous neck
cell is a precursor cell of chief cells.
#Last_modified 97.03.28-14:15
#Return_path gekufmda@med1.kufm.kagoshima-u.ac.jp
#Sequence_number   72
##End
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