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[db-086] 3B39 : ÀºÁãŦ½Ð¥é¥Ã¥È¤Î²¼¿âÂÎLHºÙ˦¤ÎÁý¿£ ¡ÝPCNA¤ÈBrdU¤È¤ÎÈæ³Ó¸¡Æ¤¡Ý

##Begin
#Z 3B39
#A ÀºÁãŦ½Ð¥é¥Ã¥È¤Î²¼¿âÂÎLHºÙ˦¤ÎÁý¿£ ¡ÝPCNA¤ÈBrdU¤È¤ÎÈæ³Ó¸¡Æ¤¡Ý
#B Proliferation of pituitary LH-cells after orchidectomy in rats studied by PCNA/LHb and BrdU/LHb double immuno-staining.
#E1 °Â¹â/¸ç
#F1 ¤ä¤¹¤¿¤«/¤µ¤È¤ë
#G1 Yasutaka/Satoru
#J1 ¶âÂô°å²ÊÂç³Ø²ò˶³ØÂè2¹ÖºÂ
#K1 ¤«¤Ê¤¶¤ï¤¤¤«¤À¤¤¤¬¤¯¤«¤¤¤Ü¤¦¤¬¤¯¤À¤¤¤Ë¤³¤¦¤¶
#L1 Department of Anatomy, Kanazawa Medical University
#E2 ÌÚÆî/Íø±É»Ò
#F2 ¤³¤ß¤Ê¤ß/¤ê¤¨¤³
#G2 Kominami/Rieko
#J2 ¶âÂô°å²ÊÂç³Ø²ò˶³ØÂè2¹ÖºÂ
#K2 ¤«¤Ê¤¶¤ï¤¤¤«¤À¤¤¤¬¤¯¤«¤¤¤Ü¤¦¤¬¤¯¤À¤¤¤Ë¤³¤¦¤¶
#L2 Department of Anatomy, Kanazawa Medical University
#E3 ë¸ý/Ë­
#F3 ¤¿¤Ë¤°¤Á/¤æ¤¿¤«
#G3 Taniguchi/Yutaka
#J3 ¶âÂô°å²ÊÂç³Ø²ò˶³ØÂè2¹ÖºÂ
#K3 ¤«¤Ê¤¶¤ï¤¤¤«¤À¤¤¤¬¤¯¤«¤¤¤Ü¤¦¤¬¤¯¤À¤¤¤Ë¤³¤¦¤¶
#L3 Department of Anatomy, Kanazawa Medical University
#E4 ´¤°æ/¹¯Ç·
#F4 ¤«¤ï¤é¤¤/¤ä¤¹¤æ¤­
#G4 Kawarai/Yasuyuki
#J4 ¶âÂô°å²ÊÂç³Ø²ò˶³ØÂè2¹ÖºÂ
#K4 ¤«¤Ê¤¶¤ï¤¤¤«¤À¤¤¤¬¤¯¤«¤¤¤Ü¤¦¤¬¤¯¤À¤¤¤Ë¤³¤¦¤¶
#L4 Department of Anatomy, Kanazawa Medical University
#D ÀºÁãŦ½Ð¤Ë¤è¤ë¥é¥Ã¥È²¼¿âÂÎLHºÙ˦¤ÎÁý¿£¤òÄêÎÌŪ¤Ë¸¡Æ¤¤¹¤ë¤¿¤á¡¢¹³LHb¹³ÂΤȺÙ˦Áý¿£¥Þ¡¼¥«¡¼¡¢Bromodeoxyuridine(BrdU)¤ÈPCNA¡¢¤½¤ì¤¾¤ì¤Î¹³ÂΤȤÎÆó½ÅÌȱÖÀ÷¿§¤Ë¤è¤ê¡¢¸÷³Ø¸²Èù¶À²¼¤ÇÍÛÀ­ºÙ˦¤Î¿ô¤ò¥«¥¦¥ó¥È¤·¤¿¡£
8½µÎð¤ÎWistar·ÏͺÀ­¥é¥Ã¥È¤ò¡¢Control(Intact¤ÈSham ope.)·², ÀºÁãŦ½Ð1¡¦3¡¦7¡¦14ÆüÌܤμ¸³·²¤Ë¡¢¤½¤ì¤¾¤ì4ɤ¤òÍѤ¤¤¿¡£BrdU(100mg/kg BW)¤ò²¼¿âÂÎŦ½Ð3»þ´ÖÁ°¤ËÊ¢¹ÐÆâ¤ËÅêÍ¿¤·¤¿¡£²¼¿âÂΤÏBouin±Õ¤Ç48»þ´Ö¸ÇÄê¤ò¹Ô¤¤¡¢2mm¤Î¥Ñ¥é¥Õ¥£¥óÀÚÊÒ¤òºîÀ®¤·¤¿¡£
·ë²Ì¡§BrdU¤ª¤è¤ÓPCNAÍÛÀ­ºÙ˦¤È¤âÀºÁãŦ½Ð7ÆüÌܤǥԡ¼¥¯¤ò¼¨¤¹¤¬¡¢ÀºÁãŦ½Ð·²¤ÈControl·²¤È¤ÎÍ­°Õº¹¤Ï¤Ê¤«¤Ã¤¿¡£Æó½ÅÌȱÖÀ÷¿§¤Î·ë²Ì¡¢BrdU, PCNAξÊý¤È¤âÀºÁãŦ½Ð3ÆüÌܤÇÍÛÀ­ºÙ˦¿ô¤Ï¥Ô¡¼¥¯¤ò¼¨¤·¤¿¡£Ã±ÆȤª¤è¤ÓÆó½Å¤ÎÌȱÖÈ¿±þ¤Î·ë²Ì¡¢PCNA¤ÏBrdU¤ÈƱÍÍ¡¢Áý¿£¥Þ¡¼¥«¡¼¤È¤·¤ÆÍ­¸ú¤Ç¤¢¤ë¤³¤È¤¬¼¨¤µ¤ì¤¿¡£
 LHbÍÛÀ­ºÙ˦¤ÏControl·²(30¡Þ2.3¸Ä)¤ËÂФ·¡¢ÀºÁãŦ½Ð·²¤Ç2Çܤ«¤é4.5ÇܤȤ¹¤Ù¤ÆÍ­°Õ¤ÊÁý²Ã¤ò¼¨¤·¤¿¡£¤³¤ì¤ËÂФ·¡¢Æó½ÅÌȱÖÈ¿±þÍÛÀ­ºÙ˦¿ô¤ÏBrdU/LHb, PCNA/LHb¤È¤âÀºÁãŦ½Ð3ÆüÌܤǺÇÂç¤È¤Ê¤ê¡¢¤½¤ì¤¾¤ì¥³¥ó¥È¥í¡¼¥ë·²¤ÎÌó10ÇÜ(0.23¸Ä¤«¤é3.54¸Ä¤Ø)¤ÈÌó50ÇÜ(0.34¸Ä¤«¤é16.02¸Ä¤Ø)¤ËÁý²Ã¤·¤¿¤¬¡¢¤½¤Î»þ¤ÎLHbÍÛÀ­ºÙ˦¤ËÂФ¹¤ëÆó½ÅÌȱÖÈ¿±þÍÛÀ­ºÙ˦¤Î³ä¹ç¤Ï¡¢BrdU¤Ç¤ÏÁ´ÂΤÎÌó3%¡¢PCNA¤Ç¤ÏÁ´ÂΤÎÌó15%¤òÀê¤á¤ë¤Î¤ß¤Ç¡¢ÀºÁãŦ½Ð¸å¤ÎLHºÙ˦¤ÎÁý²Ã¤Ï¡¢LHºÙ˦¤½¤Î¤â¤Î¤ÎºÙ˦ʬÎö¤Ë¤è¤ë³ä¹ç¤Ï¾¯¤Ê¤¯¡¢ÂçÉôʬ¤Ï̤ʬ²½¤ÊÁ°¶îºÙ˦¤«¤é¤Îʬ²½¤Ç¤¢¤ë¤È»×¤ï¤ì¤ë¡£
#M LHÍÛÀ­ºÙ˦,¥Ö¥í¥â¥Ç¥ª¥­¥·¥¦¥ê¥¸¥ó,£Ð£Ã£Î£Á,ÌȱֺÙ˦²½³Ø,¥é¥Ã¥È,¸÷³Ø¸²Èù¶À
#N LH positive cells,Bromodeoxiuridine,PCNA,Immunocytochemistry,Rat,Light microscopy
#Last_modified 97.04.01-09:12
#Return_path ankoh@kanazawa-med.ac.jp
#Sequence_number   86
##End
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